How to classify PCR machines? How many do you know?
PCR instrument, also known as amplification instrument, is a device that uses PCR to synthesize a large amount of specific tissues in vitro for the purpose of detecting DNA/RNA in various tissue analyses. The main principle of the PCR instrument is similar to that of basic measuring instruments, with high requirements for measuring elements. Once it loses control, the instrument will not work properly. Therefore, the PCR amplification instrument also needs regular maintenance, especially for PCR amplification instruments that rely on natural wind cooling.
There are many different types of PCR machines, each with its own design, price, and functional characteristics. However, their functions and application areas are the same. So, how to classify PCR machines? How many do you know?
1. Ordinary PCR instrument
A PCR instrument that can only run at a specific annealing temperature for a single PCR amplification is called a traditional PCR instrument, also known as a regular PCR instrument. Mainly to perform simple amplification of annealing temperature for the target tissue.
2. Gradient PCR instrument
A series of different annealing temperature conditions (temperature gradients) can be set for one-time PCR amplification, usually with 12 temperature gradients. Such an instrument is called a gradient PCR instrument. This instrument is mainly used to study the amplification of unknown DNA annealing temperatures, which not only saves costs but also time. Mainly used for scientific research and teaching institutions. Gradient PCR machine can also perform regular PCR amplification without setting gradients. Not many have dual slot gradients, but Lingcheng has this function.
3. In situ PCR instrument
An intracellular tissue amplification instrument used for analyzing the localization of target DNA within cells, such as the location of pathogenic tissue in cells or the location of target tissue in cells.
4. Real time fluorescence quantitative PCR instrument
Adding a fluorescence signal acquisition system and a computer analysis and processing system on the basis of a regular PCR instrument becomes a fluorescence quantitative PCR instrument. The principle of PCR amplification is the same as that of ordinary PCR instruments, except that the primers added during PCR amplification are labeled with isotopes, fluorescein, etc. The primers and fluorescent probes are used to specifically bind to the template for amplification.
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